Molecular Detection for Some Virulence Factors of Salmonella typhi from Iraqi Patients
Menan Abdul-Razaq Abdul-Khadum
College of Education for Pure Science- Diyala University, Iraq.
College of Education for Pure Science- Diyala University, Iraq.
Ali Jaffar Saleem
College of Education for Pure Science- Diyala University, Iraq.
College of Education for Pure Science- Diyala University, Iraq.
Amal Aziz Karim
Middle Technical University, College of Health and medical Technology, Research unite, Iraq.
Middle Technical University, College of Health and medical Technology, Research unite, Iraq.
Abstract
In current study, 29 clinical isolates Salmonella typhi were collected from different Private laboratories in Diyala. In addtion of phenotypic method, all isolates diagnosed as S. typhi by genotypic method was done by Polymerase Chain Reaction (PCR) technique to detection 16SrRNA gene. And detection of adhesion factors associated genes that encoding for cdtB, tviA, fimA and orfL by using specific primers which showed the prevalence in percentage 100% for all genes.
Keywords: Salmoella typhi ,16SrRNA, cdtB, tviA, fimA and orfL genes, Virulence Factors genes. ,
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