Molecular Detection of Some Adhesion Genes of Escherichia Coli Isolated from Patients with Urinary Tract Infection
Esraa Ahmed Abbas
College of Education for Pure Science - Diyala University, Iraq.
College of Education for Pure Science - Diyala University, Iraq.
Ali Jaffar Saleem
College of Education for Pure Science - Diyala University, Iraq.
College of Education for Pure Science - Diyala University, Iraq.
Abstract
In current study, 150 samples were collected from patients with urinary tract infection of both genders and all ages starting from October 2021 to January 2022 in Diyala and Baghdad governorates, Iraq. They were tested by phenotypic methods and the results showed that 73 isolates belonged to E. coli.
In addtion of phenotypic method, all isolates diagnosed by genotypic method was done by Polymerase Chain Reaction (PCR) technique to detection papE gene, Results showed that 22 isolates (30.1%) contain this gene belonging to UPEC.
Molecular detection results also showed some adhesion genes of UPEC which included (fimH, papC, sfa, and afa) its presence among the isolates was (100%), (31.81%), (100%), and (40.9%) respectively.
Keywords: E. coli, Adhesion Genes, papE gene, UPEC. ,
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