Purification and Biotechnological Potential of Laccase Produced by Aspergillus niger Using Surface Fermentation

Authors

  • Liza Dr. Ikram UL Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan Author
  • Shakir Ullah Department of Biological Sciences, National University of Medical Sciences (NUMS), Abid Majeed Road, 46000, Rawalpindi, Pakistan Author
  • Hafsa Batool Dr. Ikram UL Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan Author
  • Amna Iftikhar Dr. Ikram UL Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan Author
  • Shahnawaz Bhatti Department of Biotechnology, Federal Urdu University of Arts, Science and Technology, Pakistan Author
  • Amna waseem Dr. Ikram UL Haq Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan Author

DOI:

https://doi.org/10.48047/

Keywords:

Laccase, Aspergillus niger, Surface fermentation, Purification, Biotechnological applications

Abstract

Laccases are versatile multicopper oxidases with wide-ranging industrial and environmental applications due to their ability to oxidize diverse phenolic and non-phenolic substrates. In the present study, laccase was produced by Aspergillus niger through surface fermentation and subsequently purified to enhance its catalytic efficiency and stability. The purification process involved ammonium sulfate precipitation, dialysis, and chromatographic separation, which yielded a highly active laccase fraction with improved specific activity. The enzyme was characterized for its optimal pH, temperature stability, and substrate specificity. Results revealed that the purified laccase exhibited significant potential in the decolorization of synthetic dyes, biodegradation of phenolic pollutants, and possible applications in biobleaching and food industries. The findings demonstrate that surface fermentation by A. niger offers a cost-effective approach for laccase production, and the purified enzyme holds promise as an eco-friendly biocatalyst for industrial and environmental biotechnology.

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Published

2025-10-28

Issue

Vol. 11 No. 2 (2025): 11 (2) 2025

Section

Articles

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Copyright (c) 2025 Liza*, Shakir Ullah, Hafsa Batool, Amna Iftikhar, Shahnawaz Bhatti, Amna waseem

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How to Cite

Liza, Ullah, S., Batool, H., Iftikhar, A., Bhatti, S., & waseem, A. (2025). Purification and Biotechnological Potential of Laccase Produced by Aspergillus niger Using Surface Fermentation. History of Medicine, 11(2), 190-201. https://doi.org/10.48047/